Rivista
/
Immunologia e infezioni
/
Detección de péptidos que activan MRGPRX2 utilizando células HEK modificadas
JoVE Journal
Immunologia e infezioni
Author Produced
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells
Please note that all translations are automatically generated. Click here for the English version.

Detección de péptidos que activan MRGPRX2 utilizando células HEK modificadas

2,304 Views

•

12:38 min

•

November 06, 2021

DOI:

10.3791/62448-v

DOI:
10.3791/62448-v

•

12:38 min
November 06, 2021

•

8 Views
, ,
1Department of Chemical and Materials Engineering, Donadeo Innovation Centre for Engineering,University of Alberta, 2School of Life Science and Engineering,Southwest Jiaotong University

Summary

Automatically generated

Se describen técnicas para generar una biblioteca de péptidos cortos que pueden activar los mastocitos a través del receptor MRGPRX2. Las técnicas asociadas son fáciles, económicas y se pueden extender a otros receptores celulares.

Explore More Videos

MRGPRX2Mast CellsPeptide LibraryAlanine ScanningN-TruncatedC-TruncatedN+C-TruncatedSolid Phase Peptide SynthesisFura-2 AM Calcium Sensitive DyeGrynkiewicz EquationCalcium Signaling

Read Article

Cite this Article

Raj, S., Lu, L., Unsworth, L. D. Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells. J. Vis. Exp. (177), e62448, doi:10.3791/62448 (2021).

Download .ris file

Copy

Share Video

.

Copy