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使用麦格纳波特奥里扎伊的色度免疫沉淀测序方法对希斯通修饰分布进行全基因组分析
JoVE Journal
Genetica
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JoVE Journal Genetica
Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
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使用麦格纳波特奥里扎伊的色度免疫沉淀测序方法对希斯通修饰分布进行全基因组分析

DOI:
10.3791/62423-v

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09:25 min

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June 02, 2021

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, , , , , ,
1Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Department of Agronomy,Beijing University of Agriculture

Capitoli

  • 00:04Introduction
  • 00:39Preparation of Protoplasts from M. oryzae
  • 01:48In Vivo Crosslinking and Sonication
  • 03:22IP of Crosslinked Protein/DNA
  • 04:23Collecting and Rinsing the IP Products
  • 05:04Elution and Reverse Crosslinking of Protein/DNA Complexes
  • 05:56Purification and Recovery of DNA
  • 06:51DNA Repair and Solexa Library Construction
  • 08:23Results: Chromatin Immunoprecipitation Sequencing Method for Genome-wide Histone Modification Analysi
  • 08:53Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们提出了一个协议，以分析全基因组分布的色石修改，它可以识别新的目标基因在M.oryzae和其他丝状真菌的发病机制。

Tags

Chromatin Immunoprecipitation Sequencing (ChIP-seq)Histone ModificationsMagnaporthe OryzaeEpigenetic RegulationFungal PathogenesisGenome-wide AnalysisTranscription FactorsDNA-protein InteractionsProtoplast PreparationSonicationAgarose Gel Electrophoresis

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