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15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
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JoVE Journal Biochimica
15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

4,688 Views

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08:09 min

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April 19, 2021

DOI:

10.3791/62395-v

DOI:
10.3791/62395-v

•

08:09 min
April 19, 2021

•

16 Views
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1Department of Chemistry,Iowa State University, 2Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology,Iowa State University

Summary

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Here, a detailed description of the protocol implemented in the laboratory for acquisition and analysis of 15N relaxation dispersion profiles by solution NMR spectroscopy is provided.

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15N CPMGProtein Conformational DynamicsNMR SpectroscopyBiomolecular StructureHSQCPulse ProgramAcquisition ParametersWater SuppressionRelaxation Dispersion

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Cite this Article

Singh, A., Purslow, J. A., Venditti, V. 15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale. J. Vis. Exp. (170), e62395, doi:10.3791/62395 (2021).

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