<sup>15</sup>N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the &#181;s-ms Timescale

Aayushi Singh; Jeffrey A. Purslow; Vincenzo Venditti

doi:10.3791/62395

Journal

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Biochemie

/

¹⁵N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

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Biochemie

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JoVE Journal Biochemie

¹⁵N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

4,688 Views

•

08:09 min

•

April 19, 2021

DOI:

10.3791/62395-v

DOI:

10.3791/62395-v

•

08:09 min

April 19, 2021

•

16 Views

Aayushi Singh¹, Jeffrey A. Purslow¹, Vincenzo Venditti^1,2

¹Department of Chemistry,Iowa State University, ²Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology,Iowa State University

Summary

Here, a detailed description of the protocol implemented in the laboratory for acquisition and analysis of ¹⁵N relaxation dispersion profiles by solution NMR spectroscopy is provided.

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15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

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¹⁵N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale