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Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits (Oryctolagus cuniculus)
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits (Oryctolagus cuniculus)

Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits (Oryctolagus cuniculus)

DOI:
10.3791/62317-v

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13:00 min

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July 31, 2021

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, , ,
1Division of Laboratory Animal Resources,Duke University Medical Center, 2Department of Pathology,Duke University Medical Center, 3Department of Immunology,Duke University Medical Center, 4Duke Human Vaccine Institute,Duke University Medical Center

Capitoli

  • 00:04Introduction
  • 00:43Maternal Intranasal Immunization Study Design
  • 03:19Intranasal Immunization
  • 06:03Droplet Issues
  • 06:33Milk Collection
  • 07:49Milk Collection Demonstration
  • 10:07Representative Results
  • 12:17Summary

Summary

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This article describes and demonstrates the administration of intranasal vaccines and the collection of milk from lactating rabbits (Oryctolagus cuniculus) as a means to assess mucosal immunity in a translationally appropriate model of maternal immunization.

Tags

Maternal ImmunizationRabbit ModelIntranasal ImmunizationMilk CollectionAntibody TransferTransplacental Antibody TransferIgGIgANew Zealand White RabbitsPlacentationMaternal-fetal ImmunityNewborn ProtectionPassive Immunity

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