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Immunostaining van Whole-Mount Retinas met de CLARITY Tissue Clearing Method
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Immunostaining of Whole-Mount Retinas with the CLARITY Tissue Clearing Method
DOI:

09:01 min

March 06, 2021

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Capitoli

  • 00:04Introduction
  • 00:36Tissue Preparation
  • 03:04Immunostaining and Refractive Index Matching
  • 04:05Mounting
  • 05:30Imaging
  • 06:36Results: Improved 3D Resolution of Fine Processes of Retina by Modified CLARITY Tissue Clearing Method
  • 08:39Conclusion

Summary

Traduzione automatica

Hier presenteren we een protocol om de CLARITY-methode van de hersenweefsels aan te passen voor volledig gemonteerde retinas om de kwaliteit van standaard immunohistochemische kleuring en hoge resolutie beeldvorming van retinale neuronen en hun subcellulaire structuren te verbeteren.

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