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Mesures basées sur la fluorescence de l’échange phosphatidylsérine/phosphatidylinositol 4-phosphate entre les membranes
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Biochimica
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JoVE Journal Biochimica
Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
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Mesures basées sur la fluorescence de l’échange phosphatidylsérine/phosphatidylinositol 4-phosphate entre les membranes

3,619 Views

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08:49 min

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March 14, 2021

DOI:

10.3791/62177-v

DOI:
10.3791/62177-v

•

08:49 min
March 14, 2021

•

2 Views
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1Université Côte d’Azur, Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire

Summary

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Ici, nous décrivons des protocoles utilisant des capteurs lipidiques fluorescents et des liposomes pour déterminer si une protéine extrait et transporte de la phosphatidylsérine ou du phosphatidylinositol 4-phosphate in vitro.

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Cite this Article

Ikhlef, S., Lipp, N., Magdeleine, M., Drin, G. Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes. J. Vis. Exp. (169), e62177, doi:10.3791/62177 (2021).

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