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Biochemistry
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JoVE Journal Biochemistry
Estimation of Plant Biomass Lignin Content using Thioglycolic Acid (TGA)
 

Estimation of Plant Biomass Lignin Content using Thioglycolic Acid (TGA)

Article DOI: 10.3791/62055-v • 09:25 min • July 24th, 2021
July 24th, 2021 •
1, 2, 2
1Department of Plant and Soil Science, Texas Tech University, 2Fiber and Biopolymer Research Institute (FBRI), Department of Plant and Soil Science, Texas Tech University

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Here, we present a modified TGA method for estimation of lignin content in herbaceous plant biomass. This method estimates the lignin content by forming specific thioether bonds with lignin and presents an advantage over the Klason method, as it requires a relatively small sample for lignin content estimation.

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Plant Biomass Lignin Content Thioglycolic Acid Method Estimation Complex Heteropolymer Monolignols Cellulose Reliable Method Thioether Bonds Protocol Phases Plant Material Preparation Alcohol Insoluble Extract Isolation Lignin Precipitation Acidic Conditions Lignin Standard Curve Industrial Bamboo Lignin Estimation Process Plant Material Collection Root Separation Washing And Drying Process Labeling And Incubation Process Tissue Cutting Or Crushing Process Biomass Grinder
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