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Immunologia e infezioni

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细菌性心内膜炎小鼠模型中感染参数的内注射与定量

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis

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细菌性心内膜炎小鼠模型中感染参数的内注射与定量

10,205 Views

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07:24 min

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February 06, 2021

DOI:

10.3791/61749-v

DOI:

10.3791/61749-v

•

07:24 min

February 06, 2021

•

13 Views

Md Huzzatul Mursalin*^1,2, Erin Livingston*¹, Phillip S. Coburn^2,3, Frederick C. Miller⁴, Roger Astley^2,3, Michelle C. Callegan^1,2,3

¹Department of Microbiology and Immunology,University of Oklahoma Health Sciences Center, ²Department of Ophthalmology,Dean McGee Eye Institute, ³Dean McGee Eye Institute, ⁴Department of Cell Biology and Department of Family and Preventive Medicine,University of Oklahoma Health Sciences Center

Summary

在这里，我们描述了一种细菌性心内膜炎小鼠模型中的内注射和随后的细菌定量方法。该协议可以扩展用于测量宿主免疫反应和细菌和宿主基因表达。

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