È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis
6,090 Views
•
08:46 min
•
August 12, 2020
DOI:
Summary
Automatically generated
Infection of neonatal mice with bioluminescent E. coli O1:K1:H7 results in a septic infection with significant pulmonary inflammation and lung pathology. Here, we describe procedures to model and further study neonatal sepsis using longitudinal intravital imaging in parallel with enumeration of systemic bacterial burdens, inflammatory profiling, and lung histopathology.
Read Article
Cite this Article
Seman, B. G., Povroznik, J. M., Vance, J. K., Rawson, T. W., Robinson, C. M. A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis. J. Vis. Exp. (162), e61609, doi:10.3791/61609 (2020).
Copy