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Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish
JoVE Journal
Medicina
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JoVE Journal Medicina
Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

DOI:
10.3791/61575-v

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13:25 min

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May 15, 2021

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, , , , , ,
1Department of Immunology,University of São Paulo, 2Department of Medicine, Nephrology Division,Federal University of São Paulo

Capitoli

  • 00:00Introduction
  • 01:48Cisplatin Injection
  • 03:41Tissue Processing for Flow Cytometry
  • 05:58Tissue Processing for TUNEL Assay
  • 07:46TUNEL Assay
  • 10:26Risultati
  • 12:29Conclusion

Summary

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This protocol describes the procedures to induce Acute Kidney Injury (AKI) in adult zebrafish using cisplatin as a nephrotoxic agent. We detailed the steps to evaluate the reproducibility of the technique and two techniques to analyze inflammation and cell death in the renal tissue, flow cytometry and TUNEL, respectively.

Tags

Acute Kidney InjuryCisplatinZebrafishNephrotoxicityInflammationCell DeathFlow CytometryTUNEL AssayChemotherapyRenal ProtectionRegeneration

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