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Inhibition of Wound Epidermis Formation via Full Skin Flap Surgery During Axolotl Limb Regeneration
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Biologia
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JoVE Journal Biologia
Inhibition of Wound Epidermis Formation via Full Skin Flap Surgery During Axolotl Limb Regeneration

Inhibition of Wound Epidermis Formation via Full Skin Flap Surgery During Axolotl Limb Regeneration

DOI:
10.3791/61522-v

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04:24 min

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June 24, 2020

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1Department of Stem Cell and Regenerative Biology,Harvard University, 2Department of Molecular and Cellular Biology,Harvard University

Capitoli

  • 00:04Introduction
  • 00:43Full Skin Flap Surgical Procedure
  • 02:23Post-Operative Recovery and Care
  • 03:01Results: Histological Staining of Normal Regenerating and FSF Sutured Limbs
  • 03:40Conclusion

Summary

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This article describes how to perform a surgical method to inhibit wound epidermis formation during axolotl limb regeneration by immediately suturing full thickness skin over the amputation plane. This method allows researchers to investigate the functional roles of the wound epidermis during the early stages of limb regeneration.

Tags

AxolotlLimb RegenerationWound EpidermisFull Skin Flap SurgeryAmputationAnesthesiaSutureZeugopodBoneSulfamerazine

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