Journal
/
生物学
/
Inhibition of Wound Epidermis Formation via Full Skin Flap Surgery During Axolotl Limb Regeneration
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Inhibition of Wound Epidermis Formation via Full Skin Flap Surgery During Axolotl Limb Regeneration

Inhibition of Wound Epidermis Formation via Full Skin Flap Surgery During Axolotl Limb Regeneration

DOI:
10.3791/61522-v

•

04:24 min

•

June 24, 2020

•

1Department of Stem Cell and Regenerative Biology,Harvard University, 2Department of Molecular and Cellular Biology,Harvard University

Chapters

  • 00:04Introduction
  • 00:43Full Skin Flap Surgical Procedure
  • 02:23Post-Operative Recovery and Care
  • 03:01Results: Histological Staining of Normal Regenerating and FSF Sutured Limbs
  • 03:40Conclusion

Summary

自动翻译

自动翻译

This article describes how to perform a surgical method to inhibit wound epidermis formation during axolotl limb regeneration by immediately suturing full thickness skin over the amputation plane. This method allows researchers to investigate the functional roles of the wound epidermis during the early stages of limb regeneration.

Tags

Keywords: AxolotlLimb RegenerationWound EpidermisFull Skin Flap SurgeryAmputationAnesthesiaSutureZeugopodBoneSulfamerazine

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/61522?article_type=v

Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies
check_url/cn/61522?article_type=v

Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation
check_url/cn/61522?article_type=v

Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development
check_url/cn/61522?article_type=v

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
check_url/cn/61522?article_type=v

Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration
check_url/cn/61522?article_type=v

Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination
check_url/cn/61522?article_type=v

Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus
check_url/cn/61522?article_type=v

Methods for Skin Wounding and Assays for Wound Responses in C. elegans
check_url/cn/61522?article_type=v

Quantifying Leukocyte Egress via Lymphatic Vessels from Murine Skin and Tumors
check_url/cn/61522?article_type=v

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

Read Article