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使用初级培养的希波坎帕神经元来研究Axon初始片段的组装

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments

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使用初级培养的希波坎帕神经元来研究Axon初始片段的组装

4,832 Views

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06:53 min

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February 12, 2021

DOI:

10.3791/61411-v

DOI:

10.3791/61411-v

•

06:53 min

February 12, 2021

•

12 Views

Rui Yang^1,2,3, Vann Bennett^2,3

¹School of Basic Medical Sciences,Xi’an Jiaotong University, ²Department of Cell Biology,Duke University, ³Department of Biochemistry,Duke University

Summary

在这里，我们描述了一个协议，定量地研究海马神经元的轴向初始段（AIS）的组装和结构，由于没有一个巨大的安基林-G，缺乏预组装的 AIS。

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