Rivista
/
Neuroscienze
/
Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal Neuroscienze
Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings

Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings

DOI:
10.3791/61085-v

•

07:31 min

•

May 07, 2020

•

, , , , , ,
1Department of Neurology with Experimental Neurology,Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 2NeuroCure Cluster of Excellence,Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 3Department of Neurosurgery,Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health

Capitoli

  • 00:04Introduction
  • 00:33Solution Preparation
  • 02:01Interface Chamber Preparation
  • 02:39Preparation Area Setup
  • 03:39Tissue Slicing and Storing
  • 04:37Epileptiform Activity Recording
  • 05:59Results: Representative Epileptiform and Epileptic Burst Activity
  • 06:38Conclusion

Summary

Traduzione automatica

Traduzione automatica

The presented protocol describes the transport and preparation of resected human hippocampal tissue with the ultimate goal to use vital brain slices as a preclinical evaluation tool for potential antiepileptic substances.

Tags

Keywords: Human HippocampusElectrophysiological RecordingsAcute Brain SlicesAnti-epileptic Drug DevelopmentTemporal Lobe EpilepsyCholine ACSFCarbogenationInterface ChamberVibratome

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/61085?article_type=v

Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices
check_url/it/61085?article_type=v

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology
check_url/it/61085?article_type=v

Preparation of Acute Subventricular Zone Slices for Calcium Imaging
check_url/it/61085?article_type=v

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices
check_url/it/61085?article_type=v

Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation
check_url/it/61085?article_type=v

Paired Whole Cell Recordings in Organotypic Hippocampal Slices
check_url/it/61085?article_type=v

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings
check_url/it/61085?article_type=v

Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents
check_url/it/61085?article_type=v

Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method
check_url/it/61085?article_type=v

Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies

Read Article