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Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings
JoVE 杂志
神经科学
This content is Free Access.
JoVE 杂志 神经科学
Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings

Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings

DOI:
10.3791/61085-v

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07:31 min

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May 07, 2020

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, , , , , ,
1Department of Neurology with Experimental Neurology,Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 2NeuroCure Cluster of Excellence,Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 3Department of Neurosurgery,Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health

Chapters

  • 00:04Introduction
  • 00:33Solution Preparation
  • 02:01Interface Chamber Preparation
  • 02:39Preparation Area Setup
  • 03:39Tissue Slicing and Storing
  • 04:37Epileptiform Activity Recording
  • 05:59Results: Representative Epileptiform and Epileptic Burst Activity
  • 06:38Conclusion

Summary

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The presented protocol describes the transport and preparation of resected human hippocampal tissue with the ultimate goal to use vital brain slices as a preclinical evaluation tool for potential antiepileptic substances.

Tags

Human HippocampusElectrophysiological RecordingsAcute Brain SlicesAnti-epileptic Drug DevelopmentTemporal Lobe EpilepsyCholine ACSFCarbogenationInterface ChamberVibratome

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