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Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings

Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings

DOI:
10.3791/61085-v

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07:31 min

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May 07, 2020

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, , , , , ,
1Department of Neurology with Experimental Neurology,Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 2NeuroCure Cluster of Excellence,Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 3Department of Neurosurgery,Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health

Chapitres

  • 00:04Introduction
  • 00:33Solution Preparation
  • 02:01Interface Chamber Preparation
  • 02:39Preparation Area Setup
  • 03:39Tissue Slicing and Storing
  • 04:37Epileptiform Activity Recording
  • 05:59Results: Representative Epileptiform and Epileptic Burst Activity
  • 06:38Conclusion

Summary

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The presented protocol describes the transport and preparation of resected human hippocampal tissue with the ultimate goal to use vital brain slices as a preclinical evaluation tool for potential antiepileptic substances.

Tags

Human HippocampusElectrophysiological RecordingsAcute Brain SlicesAnti-epileptic Drug DevelopmentTemporal Lobe EpilepsyCholine ACSFCarbogenationInterface ChamberVibratome

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