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マウス副腎のマルチプレックス免疫染色のためのマイクロウェーブおよび蛍光色素-Tyramide - 同じ宿主種からの非共役一次抗体を使用
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JoVE Journal Biologia dello sviluppo
Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species
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マウス副腎のマルチプレックス免疫染色のためのマイクロウェーブおよび蛍光色素-Tyramide - 同じ宿主種からの非共役一次抗体を使用

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07:37 min

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February 21, 2020

DOI:

10.3791/60868-v

DOI:
10.3791/60868-v

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07:37 min
February 21, 2020

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1 Views
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1College of Animal Science & Technology,Henan University of Science and Technology, 2Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine,Auburn University

Tags

Multiplex ImmunostainingUnconjugated Primary AntibodiesFormalin-fixed Paraffin-embeddedSodium CitratePBSTBlocking SolutionSecondary AntibodyStreptavidin Horseradish PeroxidaseFluorophore-tyramide

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Lyu, Q., Zheng, H. S., Laprocina, K., Huang, C. J. Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species. J. Vis. Exp. (156), e60868, doi:10.3791/60868 (2020).

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