Rivista
/
Biologia dello sviluppo
/
微挥和荧光素-提拉美化物，用于小鼠肾上腺的多重免疫染色 – 使用来自同一宿主物种的未结合原抗体
JoVE Journal
Biologia dello sviluppo
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species
Please note that all translations are automatically generated. Click here for the English version.

微挥和荧光素-提拉美化物，用于小鼠肾上腺的多重免疫染色 – 使用来自同一宿主物种的未结合原抗体

6,699 Views

•

07:37 min

•

February 21, 2020

DOI:

10.3791/60868-v

DOI:
10.3791/60868-v

•

07:37 min
February 21, 2020

•

2 Views
, , ,
1College of Animal Science & Technology,Henan University of Science and Technology, 2Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine,Auburn University

Summary

Automatically generated

利用来自同一宿主物种的初级抗体，已经建立了几种不同的多路免疫染色方法。在这里，我们描述了在正式固定石蜡嵌入小鼠肾上腺部分进行多倍免疫染色期间，使用微波介导的抗体剥离和荧光素-酪酰胺来阻止抗体的交叉反应。

Explore More Videos

Multiplex ImmunostainingUnconjugated Primary AntibodiesFormalin-fixed Paraffin-embeddedSodium CitratePBSTBlocking SolutionSecondary AntibodyStreptavidin Horseradish PeroxidaseFluorophore-tyramide

Read Article

Cite this Article

Lyu, Q., Zheng, H. S., Laprocina, K., Huang, C. J. Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species. J. Vis. Exp. (156), e60868, doi:10.3791/60868 (2020).

Download .ris file

Copy

Share Video

.

Copy