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08:06 min
February 14, 2020
DOI:
10.3791/60809-v
我们描述了牙髓(DP)原细胞的分离、分散和电镀,这些细胞在覆盖的透井过滤器上培养三叉(TG)神经元。DP细胞的细胞反应可以通过免疫荧光或RNA/蛋白质分析进行分析。神经元标记物与共聚焦显微镜的免疫荧光允许分析神经质外生长反应。
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Barkley, C., Serra, R., Peters, S. B. A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals. J. Vis. Exp. (156), e60809, doi:10.3791/60809 (2020).
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