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Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales
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Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales

Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales

6,260 Views

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12:32 min

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November 25, 2020

DOI:

10.3791/60701-v

DOI:
10.3791/60701-v

•

12:32 min
November 25, 2020

•

5 Views
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1Stream Biofilm and Ecosystem Research Laboratory,École Polytechnique Fédérale de Lausanne, 2Institute of Earth Sciences,University of Lausanne, CH-1015

Summary

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Breakthrough curves (BTCs) are efficient tools to study the transport of bacteria in porous media. Here we introduce tools based on fluidic devices in combination with microscopy and flow cytometric counting to obtain BTCs.

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Microfluidic DevicesMicroscopyFlow CytometryBacterial TransportPorous MediaSpatial ScalesBioremediationContaminationDisease SpreadMathematical ModelsSingle CellPopulationMicrobial CommunityPDMSSU-8 PhotolithographyPorous GeometryFluidic DevicesAutomated DispenserLaboratory-scale Column StudiesField Surveys

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Scheidweiler, D., De Anna, P., Battin, T. J., Peter, H. Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales. J. Vis. Exp. (165), e60701, doi:10.3791/60701 (2020).

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