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Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales
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November 25, 2020
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Breakthrough curves (BTCs) are efficient tools to study the transport of bacteria in porous media. Here we introduce tools based on fluidic devices in combination with microscopy and flow cytometric counting to obtain BTCs.
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Microfluidic DevicesMicroscopyFlow CytometryBacterial TransportPorous MediaSpatial ScalesBioremediationContaminationDisease SpreadMathematical ModelsSingle CellPopulationMicrobial CommunityPDMSSU-8 PhotolithographyPorous GeometryFluidic DevicesAutomated DispenserLaboratory-scale Column StudiesField Surveys
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Scheidweiler, D., De Anna, P., Battin, T. J., Peter, H. Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales. J. Vis. Exp. (165), e60701, doi:10.3791/60701 (2020).
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