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Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection

Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection

DOI:
10.3791/60680-v

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09:34 min

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March 18, 2020

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, , , ,
1Centro de Terapia Celular e Molecular (CTCMol),Universidade Federal de São Paulo (UNIFESP), 2Departamento de Ciências Biológicas, Centro de Terapia Celular e Molecular (CTCMol),Universidade Federal de São Paulo (UNIFESP)

Capitoli

  • 00:04Introduction
  • 01:31Glial Cells Extraction, Maintenance, and Dissociation
  • 07:28Results: Astrocytes and Microglia Infection
  • 08:32Conclusion

Summary

Traduzione automatica

Traduzione automatica

The overall goal of this protocol is to instruct how to extract, maintain, and dissociate murine astrocyte and microglia cells from the central nervous system, followed by infection with protozoa parasites.

Tags

Astrocyte IsolationMicroglia IsolationGlial Cell ExtractionCentral Nervous SystemImmunological ContextAlzheimer’sParkinson’sProtozoa ParasiteVirus Infection

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