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Developmental Biology

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JoVE Journal Developmental Biology
Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
 

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Article DOI: 10.3791/60455-v • 10:25 min • December 12th, 2019
December 12th, 2019 •
1, 1,2,3,4
1Institute for Frontier Life and Medical Sciences, Kyoto University, 2Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, 3Kyoto University Graduate School of Medicine, 4Kyoto University Graduate School of Biostudies

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Neural stem/progenitor cells exhibit various expression dynamics of Notch signaling components that lead to different outcomes of cellular events. Such dynamic expression can be revealed by real-time monitoring, not by static analysis, using a highly sensitive bioluminescence imaging system that enables visualization of rapid changes in gene expressions.

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Real-time Bioluminescence Imaging Notch Signaling Dynamics Murine Neurogenesis Gene Expression Dynamics Cell Proliferation Differentiation Neural Stem Cells In Vitro In Vivo Live Imaging Hiromi Shimojo Dll1 F Luciferase Reporter Pedal Reflex Embryonic Day 12.5 To 14.5 Pregnant Mouse Abdominal Incision Uterine Horn Extraction Micro Capillary Injection Blue Dye Observance Electroporation Positively Charged Electrode
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