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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
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发育生物学
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JoVE 杂志 发育生物学
Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

7,356 Views

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10:25 min

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December 12, 2019

DOI:

10.3791/60455-v

DOI:
10.3791/60455-v

•

10:25 min
December 12, 2019

•

2 Views
,
1Institute for Frontier Life and Medical Sciences,Kyoto University, 2Institute for Integrated Cell-Material Sciences (iCeMS),Kyoto University, 3Kyoto University Graduate School of Medicine, 4Kyoto University Graduate School of Biostudies

Summary

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Neural stem/progenitor cells exhibit various expression dynamics of Notch signaling components that lead to different outcomes of cellular events. Such dynamic expression can be revealed by real-time monitoring, not by static analysis, using a highly sensitive bioluminescence imaging system that enables visualization of rapid changes in gene expressions.

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Cite this Article

Shimojo, H., Kageyama, R. Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis. J. Vis. Exp. (154), e60455, doi:10.3791/60455 (2019).

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