/

/

मानव Pluripotent स्टेम सेल में CRISPR-CAS9 का उपयोग कर परिभाषित जीनोमिक संशोधनों का उत्पादन

JoVE Journal
Genetica

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Genetica

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Please note that all translations are automatically generated. Click here for the English version.

मानव Pluripotent स्टेम सेल में CRISPR-CAS9 का उपयोग कर परिभाषित जीनोमिक संशोधनों का उत्पादन

7,996 Views

•

09:04 min

•

September 25, 2019

DOI:

10.3791/60085-v

DOI:

10.3791/60085-v

•

09:04 min

September 25, 2019

•

5 Views

Fabian L. Cardenas-Diaz*¹, Jean Ann Maguire*¹, Paul Gadue^2,3, Deborah L. French^2,3

¹Center for Cellular and Molecular Therapeutics,The Children’s Hospital of Philadelphia, ²Department of Pathology and Laboratory Medicine,The Children’s Hospital of Philadelphia, ³Department of Pathology and Laboratory Medicine,University of Pennsylvania

Tags

CRISPR-Cas9 Human Pluripotent Stem Cells Isogenic Lines Heterozygous Mutations Gene Function Disease Modeling Colony Picking Fluorescence-activated Cell Sorting MEFs ROCK Inhibitor

Read Article