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Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption
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JoVE Journal Biochimica
Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

5,378 Views

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09:20 min

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October 04, 2019

DOI:

10.3791/60034-v

DOI:
10.3791/60034-v

•

09:20 min
October 04, 2019

•

1 Views
,
1Department of Mechanical and Aerospace Engineering,University of Central Florida, 2Burnett School of Biomedical Sciences, College of Medicine,University of Central Florida

Summary

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Here, we present a mechanics-based protocol to disrupt the gap junction connexin 43 and measure the subsequent impact this has on endothelial biomechanics via observation of tractions and intercellular stresses.

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Islam, M. M., Steward, Jr., R. L. Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption. J. Vis. Exp. (152), e60034, doi:10.3791/60034 (2019).

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