ジャーナル
/
生化学
/
Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption
JoVE Journal
生化学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 生化学
Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

5,381 Views

•

09:20 min

•

October 04, 2019

DOI:

10.3791/60034-v

DOI:
10.3791/60034-v

•

09:20 min
October 04, 2019

•

1 Views
,
1Department of Mechanical and Aerospace Engineering,University of Central Florida, 2Burnett School of Biomedical Sciences, College of Medicine,University of Central Florida

概要

Automatically generated

Here, we present a mechanics-based protocol to disrupt the gap junction connexin 43 and measure the subsequent impact this has on endothelial biomechanics via observation of tractions and intercellular stresses.

Explore More Videos

Endothelial BiomechanicsConnexin 43Cell-cell JunctionsCell-generated Mechanical ForcesTractionsIntercellular StressorsOrgan-on-chipIn Vitro Drug DeliveryBind-silane SolutionHydrogelFluorescent BeadsAcrylamideBisacrylamideAmmonium PersulphateTEMED

Read Article

Cite this Article

Islam, M. M., Steward, Jr., R. L. Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption. J. Vis. Exp. (152), e60034, doi:10.3791/60034 (2019).

Download .ris file

Copy

Share Video

.

Copy