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Kvantifiering av proteininteraktion Nätverksdynamik med hjälp av multiplexerade Co-immunoprecipitation
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1Center for Integrative Brain Research,Seattle Children’s Research Institute, 2Graduate Program in Neuroscience,University of Washington, 3Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Department of Medicine,Harvard Medical School, 4Broad Institute of Harvard and MIT, 5Department of Mathematics,University of Houston, 6Department of Molecular Microbiology and Immunology, School of Medicine,University of Missouri, 7Department of Surgery, School of Medicine,University of Missouri, 8Department Bioengineering, College of Engineering,University of Missouri, 9Department of Pediatrics,University of Washington
Capitoli
- 00:04Titolo
- 00:59Quantitative Multiplex Immunoprecipitation (Day 1)
- 02:19Running the Assay (Day 2)
- 04:40Data Analysis
- 06:10Positive Hits and Visualization
- 06:57Results: Representative Protein Interaction Network Dynamic Quantification
- 07:20Conclusion
Kvantitativ multiplex immunoprecipitation (QMI) använder flödescytometri för känslig detektion av skillnader i överflödet av riktade protein-protein interaktioner mellan två prover. QMI kan utföras med en liten mängd biomaterial, kräver inte genetiskt modifierade taggar, och kan anpassas för alla tidigare definierade protein interaktion nätverk.
