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Journal / Biochemistry / Quantification of Protein Interaction Network…
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Biochemistry

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JoVE Journal Biochemistry
Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
 

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Article DOI: 10.3791/60029-v • 07:57 min • August 21st, 2019
August 21st, 2019 •
1,2, 3,4, 5, 6,7,8, 1,2,9
1Center for Integrative Brain Research, Seattle Children's Research Institute, 2Graduate Program in Neuroscience, University of Washington, 3Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School, 4Broad Institute of Harvard and MIT, 5Department of Mathematics, University of Houston, 6Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, 7Department of Surgery, School of Medicine, University of Missouri, 8Department Bioengineering, College of Engineering, University of Missouri, 9Department of Pediatrics, University of Washington

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Summary
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Quantitative Multiplex Immunoprecipitation (QMI) uses flow cytometry for sensitive detection of differences in the abundance of targeted protein-protein interactions between two samples. QMI can be performed using a small amount of biomaterial, does not require genetically engineered tags, and can be adapted for any previously defined protein interaction network.

Tags

Quantification Protein Interaction Network Dynamics Multiplexed Co-Immunoprecipitation QMI Binary Interactions Protein Interaction Network Co-associations Bioinformatics Techniques Signal Transduction Systems Assay Development QMI Panel Network Scale Data Overnight Assay Pipetting Sample Preparation Immunoprecipitation Lysing Detergent Protease Inhibitors Phosphatase Inhibitors Incubation On Ice Spin Down BCA Assay Protein Concentration Determination Magnetic Bead Master Mix Magnetic Separation Fly P Buffer
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