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多重共免疫沈殿を用いたタンパク質相互作用ネットワークダイナミクスの定量化
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August 21, 2019
DOI:
1Center for Integrative Brain Research,Seattle Children’s Research Institute, 2Graduate Program in Neuroscience,University of Washington, 3Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Department of Medicine,Harvard Medical School, 4Broad Institute of Harvard and MIT, 5Department of Mathematics,University of Houston, 6Department of Molecular Microbiology and Immunology, School of Medicine,University of Missouri, 7Department of Surgery, School of Medicine,University of Missouri, 8Department Bioengineering, College of Engineering,University of Missouri, 9Department of Pediatrics,University of Washington
Summary
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定量多重免疫沈殿(QMI)は、2つのサンプル間の標的タンパク質相互作用の豊富さの違いを敏感に検出するためにフローサイトメトリーを使用します。QMIは、少量の生体材料を用いて行うことができ、遺伝子組み換えタグを必要とせず、以前に定義されたタンパク質相互作用ネットワークに適応させることができる。
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Cite this Article
Brown, E. A., Neier, S. C., Neuhauser, C., Schrum, A. G., Smith, S. E. Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation. J. Vis. Exp. (150), e60029, doi:10.3791/60029 (2019).
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