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在自身免疫糖尿病模型中高效生成抗原特异性原小鼠细胞毒性T细胞细胞细胞，用于功能测试
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model
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在自身免疫糖尿病模型中高效生成抗原特异性原小鼠细胞毒性T细胞细胞细胞，用于功能测试

DOI:
10.3791/59985-v

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11:31 min

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August 16, 2019

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, , , , , ,
1Barbara Davis Center for Childhood Diabetes,University of Colorado Denver, 2Department of Medicine, Endocrinology, Diabetes & Metabolism,Baylor College of Medicine, 3Scientific Center,Shandong Provincial Hospital affiliated to Shandong University

Capitoli

  • 00:04Titolo
  • 01:00Transfection of Viral Producer Cells
  • 03:34Primary CD8 T Cell Isolation and Activation
  • 07:10Transduction of Activated CD8 T Cells
  • 09:29Results: Analysis of Transduced CD8 CAR-T Cells
  • 10:42Conclusion

Summary

Traduzione automatica

Traduzione automatica

本文介绍了一种用于生成抗原特异性CD8 T细胞及其在体外扩张的协议，目的是产生大量功能性T细胞，用于体外和体内。

Tags

Antigen-specificPrimary MouseCytotoxic T CellsAutoimmune DiabetesHigh-efficiencyTransductionCAR T CellsCancerImmune CellsPhoenix CellsTransfectionReduced Serum MediumPackaging PlasmidVirusIL-2

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