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High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model
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Immunology and Infection
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JoVE Journal Immunology and Infection
High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model

High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model

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7,648 Views

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11:31 min

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August 16, 2019

DOI:

10.3791/59985-v

DOI:
10.3791/59985-v

•

11:31 min
August 16, 2019

•

2 Views
, , , , , ,
1Barbara Davis Center for Childhood Diabetes,University of Colorado Denver, 2Department of Medicine, Endocrinology, Diabetes & Metabolism,Baylor College of Medicine, 3Scientific Center,Shandong Provincial Hospital affiliated to Shandong University

Summary

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This article describes a protocol for the generation of antigen-specific CD8 T cells, and their expansion in vitro, with the aim of yielding high numbers of functional T cells for use in vitro and in vivo.

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Cite this Article

Davidson, H. W., Cepeda, J. R., Sekhar, N. S., Han, J., Gao, L., Sosinowski, T., Zhang, L. High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model. J. Vis. Exp. (150), e59985, doi:10.3791/59985 (2019).

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