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抗体喂养方法研究分离原发性希马培养物中的谷氨酸受体贩运
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Neuroscienze
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JoVE Journal Neuroscienze
An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures
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抗体喂养方法研究分离原发性希马培养物中的谷氨酸受体贩运

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08:13 min

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August 02, 2019

DOI:

10.3791/59982-v

DOI:
10.3791/59982-v

•

08:13 min
August 02, 2019

•

2 Views
, , ,
1Department of Pharmacology, Feinberg School of Medicine,Northwestern University, 2Department of Cellular Neurophysiology,Institute of Physiology CAS

Summary

Automatically generated

本文提出了一种研究分离原发海马培养物的谷氨酸受体(GluR)贩运的方法。使用抗体喂养方法将内源性或过度表达的受体与药理学方法相结合,该方法允许通过调节来识别调节GluR表面表达的分子机制内部化或回收过程。

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Cite this Article

Chiu, A. M., Barse, L., Hubalkova, P., Sanz-Clemente, A. An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures. J. Vis. Exp. (150), e59982, doi:10.3791/59982 (2019).

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