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マウスの神経幹細胞と脳における5-ヒドロキシメチルシトシンの検出
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Neuroscienze
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JoVE Journal Neuroscienze
The Detection of 5-Hydroxymethylcytosine in Neural Stem Cells and Brains of Mice
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マウスの神経幹細胞と脳における5-ヒドロキシメチルシトシンの検出

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08:03 min

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September 19, 2019

DOI:

10.3791/59950-v

DOI:
10.3791/59950-v

•

08:03 min
September 19, 2019

•

1 Views
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1The Children’s Hospital, School of Medicine,Zhejiang University, 2The Institute of Translational Medicine, School of Medicine,Zhejiang University

Summary

Automatically generated

ここでは、細胞および脳組織中の5-ヒドロキシメチルシトシンを検出するプロトコルを提示し、免疫蛍光染色およびDNAドットブロット法を利用する。

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Cite this Article

Zhuang, Y., Chen, J., Xu, W., Shu, Q., Li, X. The Detection of 5-Hydroxymethylcytosine in Neural Stem Cells and Brains of Mice. J. Vis. Exp. (151), e59950, doi:10.3791/59950 (2019).

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