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In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
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In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
Capitoli
- 00:04Titolo
- 00:42DNA Template Preparation by PCR for In Vitro Transcription
- 03:20mRNA Generation by In Vitro Transcription
- 04:22Cells Transfection with mRNA
- 06:04Luciferase Activity Measurement
- 07:29Results: Translation Efficiency of a Fluc mRNA Determined by Luciferase Reporter Assay
- 08:21Conclusion
We present a protocol to study mRNA translation regulation in poxvirus-infected cells using in vitro Transcribed RNA-based luciferase reporter assay. The assay can be used for studying translation regulation by cis-elements of an mRNA, including 5’-untranslated region (UTR) and 3’-UTR. Different translation initiation modes can also be examined using this method.
