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Gecontroleerde corticale impact model van muis hersenletsel met therapeutische transplantatie van menselijk geïnduceerde pluripotente stamcel-afgeleide neurale cellen
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells
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Gecontroleerde corticale impact model van muis hersenletsel met therapeutische transplantatie van menselijk geïnduceerde pluripotente stamcel-afgeleide neurale cellen

10,336 Views

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09:29 min

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July 10, 2019

DOI:

10.3791/59561-v

DOI:
10.3791/59561-v

•

09:29 min
July 10, 2019

•

3 Views
, ,
1Center for Neuroscience and Regenerative Medicine,Uniformed Services University, 2Department of Anatomy, Physiology, and Genetics,Uniformed Services University, 3Graduate Program in Neuroscience,Uniformed Services University

Summary

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Dit protocol demonstreert methodologieën voor een muismodel van open-schedel traumatisch hersenletsel en transplantatie van gekweekte menselijke geïnduceerde pluripotente stamcel-afgeleide cellen in de wond site. Gedrags-en histologische tests van uitkomsten van deze procedures worden ook in het kort beschreven.

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Cite this Article

Furmanski, O., Nieves, M. D., Doughty, M. L. Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells. J. Vis. Exp. (149), e59561, doi:10.3791/59561 (2019).

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