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Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells

Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells

DOI:
10.3791/59561-v

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09:29 min

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July 10, 2019

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, ,
1Center for Neuroscience and Regenerative Medicine,Uniformed Services University, 2Department of Anatomy, Physiology, and Genetics,Uniformed Services University, 3Graduate Program in Neuroscience,Uniformed Services University

Kapitel

  • 00:04Titel
  • 00:52Craniectomy
  • 02:49Controlled Cortical Impact
  • 03:47Stereotaxic Transplantation of Cell Suspension
  • 06:38Sensorimotor Integration Adhesive Tape Removal Test
  • 07:38Results: Representative Sensorimotor Integration and Human Cell Grafting After Brain Injury
  • 08:32Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol demonstrates methodologies for a mouse model of open-skull traumatic brain injury and transplantation of cultured human induced pluripotent stem cell-derived cells into the injury site. Behavioral and histologic tests of outcomes from these procedures are also described in brief.

Tags

Controlled Cortical ImpactMouse Brain InjuryHuman Induced Pluripotent Stem CellNeural Cell TransplantationCraniectomyStereotaxic FrameImpactor ProbeRotary Micro MotorBurr Drill BitBone Flap

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