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Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
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Biochimica
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JoVE Journal Biochimica
Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

DOI:
10.3791/59438-v

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08:13 min

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April 09, 2019

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, , , , , ,
1Centre for Discovery Brain Sciences,University of Edinburgh, 2Euan MacDonald Centre for Motor Neurone Disease Research,University of Edinburgh

Capitoli

  • 00:04Titolo
  • 00:34Protein Extraction
  • 01:15Quantification and Normalization of Protein Concentration
  • 02:22Protein Sample Gel Electrophoresis and Protein Transfer
  • 04:03Total Protein Staining
  • 05:26Image Acquisition and Western Blot Analysis
  • 06:39Risultati
  • 07:32Conclusion

Summary

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This method describes a robust and reproducible approach for the comparison of protein levels in different tissues and at different developmental timepoints using a standardized quantitative western blotting approach.

Tags

Protein LevelsQuantitative Western BlottingStandardized ProtocolDevelopmental AnalysisFluorescent Total Protein StainInternal Loading ControlProtein ExtractionProtein QuantificationElectrophoresisGel LoadingBetween-membrane Normalization

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