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Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
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生物化学
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JoVE 杂志 生物化学
Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

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08:13 min

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April 09, 2019

DOI:

10.3791/59438-v

DOI:
10.3791/59438-v

•

08:13 min
April 09, 2019

•

16 Views
, , , , , ,
1Centre for Discovery Brain Sciences,University of Edinburgh, 2Euan MacDonald Centre for Motor Neurone Disease Research,University of Edinburgh

Summary

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This method describes a robust and reproducible approach for the comparison of protein levels in different tissues and at different developmental timepoints using a standardized quantitative western blotting approach.

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Huang, Y., van der Hoorn, D., Ledahawsky, L. M., Motyl, A. A. L., Jordan, C. Y., Gillingwater, T. H., Groen, E. J. N. Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting. J. Vis. Exp. (146), e59438, doi:10.3791/59438 (2019).

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