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Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells

Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells

8,843 Views

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12:08 min

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May 17, 2019

DOI:

10.3791/59370-v

DOI:
10.3791/59370-v

•

12:08 min
May 17, 2019

•

3 Views
, , , , , , , , , , ,
1Department of Dermatology,Yale University School of Medicine, 2Dermatology Department,University of Rome Tor Vergata, 3Department of Pathology,Yale University School of Medicine

Summary

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The transimmunization (TI) device or plate and related protocols have been developed to replicate the key features of extracorporeal photochemotherapy (ECP), in an experimental setting, allowing for production of physiologically activated, tunable dendritic cells (DCs) for cancer immunotherapy.

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Ventura, A., Vassall, A., Yurter, A., Robinson, E., Filler, R., Hanlon, D., Meeth, K., Ezaldein, H., Girardi, M., Sobolev, O., Bosenberg, M. W., Edelson, R. L. Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells. J. Vis. Exp. (147), e59370, doi:10.3791/59370 (2019).

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