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Immunology and Infection

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JoVE Journal Immunology and Infection
Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells
 

Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells

Article DOI: 10.3791/59370-v • 12:08 min • May 17th, 2019
May 17th, 2019 •
*1,2, *1, 1, 1, 1, 1, 1, 1, 1, 1, 1,3, 1
1Department of Dermatology, Yale University School of Medicine, 2Dermatology Department, University of Rome Tor Vergata, 3Department of Pathology, Yale University School of Medicine
* These authors contributed equally

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résumé
Automatic Translation

The transimmunization (TI) device or plate and related protocols have been developed to replicate the key features of extracorporeal photochemotherapy (ECP), in an experimental setting, allowing for production of physiologically activated, tunable dendritic cells (DCs) for cancer immunotherapy.

Tags

Physiologic Immunogenic Dendritic Cells PhDC Antigen-presenting Cells Efficient Method Platelet Signaling Monocyte-to-DC Conversion Clinical Use Cytokine Methods Anti-tumor T Cell Responses Dendritic Cell Physiology Therapeutic Anti-cancer Immunity Personalized Antimicrobial Vaccination Experimental System Plastic Chamber Coating 8-MOP/UVA Treatment Flow Conditions Release Of Monocytes Peripheral Blood Mononuclear Cells Lymphocyte Isolation Medium
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