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Purification and Transplantation of Myogenic Progenitor Cell Derived Exosomes to Improve Cardiac Function in Duchenne Muscular Dystrophic Mice
JoVE Journal
Genetica
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JoVE Journal Genetica
Purification and Transplantation of Myogenic Progenitor Cell Derived Exosomes to Improve Cardiac Function in Duchenne Muscular Dystrophic Mice

Purification and Transplantation of Myogenic Progenitor Cell Derived Exosomes to Improve Cardiac Function in Duchenne Muscular Dystrophic Mice

DOI:
10.3791/59320-v

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08:13 min

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April 10, 2019

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, , , , ,
1Vascular Biology Center, Medical College of Georgia,Augusta University, 2Renji Hospital, School of Medicine,Shanghai Jiaotong University

Capitoli

  • 00:00Titolo
  • 01:24Isolation and Purification of MPC-derived Exosomes
  • 03:10Intramyocardial Exosome Delivery
  • 04:49Echocardiography
  • 05:50Results: Analysis of Cardiac Function in Duchenne Muscular Dystrophic Mice After the Transplantation of Myogenic Progenitor Cell Derived Exosomes
  • 07:19Conclusion

Summary

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Here, we present a protocol to transiently improve cardiac function in Duchenne muscular dystrophy mice by transplanting exosomes derived from normal myogenic progenitor cells.

Tags

Duchenne Muscular DystrophyExosomesMyogenic Progenitor CellsCardiac FunctionEchocardiographyUltracentrifugationIntramyocardial Transplantation

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