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In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function
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Ricerca sul cancro
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JoVE Journal Ricerca sul cancro
In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

11,124 Views

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08:04 min

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February 27, 2019

DOI:

10.3791/59275-v

DOI:
10.3791/59275-v

•

08:04 min
February 27, 2019

•

8 Views
, , , , ,
1Department of Hematology & Hematopoietic Cell Transplantation, T Cell Therapeutics Research Laboratory,City of Hope Beckman Research Institute and Medical Center, 2Irell and Manella Graduate School of Biological Sciences,City of Hope Beckman Research Institute and Medical Center

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Here, we describe an in vitro co-culture method to recursively challenge tumor-targeted T cells, which allows for phenotypic and functional analysis of antitumor T cell activity.

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Cite this Article

Wang, D., Starr, R., Alizadeh, D., Yang, X., Forman, S. J., Brown, C. E. In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function. J. Vis. Exp. (144), e59275, doi:10.3791/59275 (2019).

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