Journal
/
Recherche en cancérologie
/
In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

DOI:
10.3791/59275-v

•

08:04 min

•

February 27, 2019

•

, , , , ,
1Department of Hematology & Hematopoietic Cell Transplantation, T Cell Therapeutics Research Laboratory,City of Hope Beckman Research Institute and Medical Center, 2Irell and Manella Graduate School of Biological Sciences,City of Hope Beckman Research Institute and Medical Center

Chapitres

  • 00:04Titre
  • 00:54Tumor Cell: T Cell Co-culture Setup and Tumor Cell Rechallenge
  • 02:37Sample Harvest for Flow Cytometric Analysis
  • 04:12Functional and Phenotypic Chimeric Antigen Receptor (CAR) T Cell Analysis
  • 05:14Results: Representative CD4+ and CD8+ CAR T Cell Phenotypes After Repetitive Challenge
  • 07:30Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we describe an in vitro co-culture method to recursively challenge tumor-targeted T cells, which allows for phenotypic and functional analysis of antitumor T cell activity.

Tags

In VitroTumor CellRechallengeChimeric Antigen ReceptorCAR T CellsAnti-tumor FunctionGlioblastomaTumorspheresCo-cultureHigh Throughput ScreeningCell DissociationCell SuspensionCell CultureSupernatantTrypsin EDTA

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization

A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia

Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity

In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells

Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography

Evaluation of the In vivo Antitumor Activity of Polyanhydride IL-1α Nanoparticles

Read Article