Rivista
/
Biochimica
/
Imaging of Extracellular Vesicles by Atomic Force Microscopy
JoVE Journal
Biochimica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biochimica
Imaging of Extracellular Vesicles by Atomic Force Microscopy

Imaging of Extracellular Vesicles by Atomic Force Microscopy

DOI:
10.3791/59254-v

•

10:11 min

•

September 11, 2019

•

,
1Department of Chemical Engineering,University of Utah, 2The Nano Institute of Utah,University of Utah, 3Center for Photonics and Quantum Materials,Skolkovo Institute of Science and Technology, 4Biopharmaceutical Cluster ‘Northern’,Moscow Institute of Physics and Technology

Capitoli

  • 00:06Titolo
  • 01:03Surface Fixation of Extracellular Vesicles
  • 03:42AFM Imaging
  • 05:28Image Analysis
  • 07:55Results: Exosome Surface Fixation and Analysis
  • 09:30Conclusion

Summary

Traduzione automatica

Traduzione automatica

A step-by-step procedure is described for label-free immobilization of exosomes and extracellular vesicles from liquid samples and their imaging by atomic force microscopy (AFM). The AFM images are used to estimate the size of the vesicles in the solution and characterize other biophysical properties. 

Tags

Keywords: Extracellular VesiclesAtomic Force MicroscopyAFM ImagingElectrostatic ImmobilizationMica SurfaceHydrated SamplesDesiccated SamplesCryoTEMNanoparticle Tracking Analysis

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/59254?article_type=v

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
check_url/it/59254?article_type=v

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
check_url/it/59254?article_type=v

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging
check_url/it/59254?article_type=v

Isolation of Tissue Extracellular Vesicles from the Liver
check_url/it/59254?article_type=v

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
check_url/it/59254?article_type=v

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
check_url/it/59254?article_type=v

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
check_url/it/59254?article_type=v

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
check_url/it/59254?article_type=v

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
check_url/it/59254?article_type=v

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Read Article