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单分子水平标记均匀性的全蛋白质定量
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Biochimica
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JoVE Journal Biochimica
Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
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单分子水平标记均匀性的全蛋白质定量

DOI:
10.3791/59199-v

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08:29 min

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April 19, 2019

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, ,
1Laboratory for Cell Systems Control, RIKEN Center for Biosystems Dynamics Research,RIKEN, 2Laboratoire de Biomatériaux et Bioimagerie,INSERM, 3PRESTO, Japan Science and Technology Agency

Capitoli

  • 00:04Titolo
  • 01:33Cell Lysis and Fluorescent Labeling
  • 03:26Proteome Separation and Recovery
  • 03:51Microscope Coverslip Preparation
  • 05:46Observation with Single Molecule Fluorescence Microscopy
  • 06:07Results: Labeling Homogeneity at the Single Molecule Level
  • 07:33Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里, 我们提出了一个协议, 以评估标签同质性的每个蛋白质物种在一个复杂的蛋白质样本在单分子水平。

Tags

Protein QuantificationSingle-molecule AnalysisFluorescent LabelingHeterogeneityMolecular Concentration AssaysDiagnostic MethodsProtein Species AnalysisData ReproducibilityLysing BufferTween-20DTTCHAPSCy3 NHS Ester DyeHEPES-NaOHBiotin-2-amineEDC

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