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Journal / Immunology and Infection / A High-throughput Shigella-specific Bactericidal…
JoVE Journal
Immunology and Infection

This Contenuto is Open Access.

JoVE Journal Immunology and Infection
A High-throughput Shigella-specific Bactericidal Assay
 

A High-throughput Shigella-specific Bactericidal Assay

Article DOI: 10.3791/59164-v • 06:11 min • February 27th, 2019
February 27th, 2019 •
1, 2, 1, 2
1Department of Enteric Infections, Bacterial Diseases Branch, Walter Reed Army Institute of Research, 2University of Alabama at Birmingham

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Here we present a protocol to measure Shigellacidal activity of antibodies in serum. Serum is mixed with bacteria and exogenous complement, incubated, and the reaction mixture is plated on agar plates. Viable bacteria form colonies which are counted, using an automated colony enumerator, and used to determine the bactericidal titer.

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High-throughput Shigella-specific Bactericidal Assay Antibodies Immunization Infection Immune Responses Bactericidal Activity High Throughput Analysis Assay Plate Assay Buffer Test Samples Serial Dilutions Multi-channel Pipette
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