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A High-throughput Shigella-specific Bactericidal Assay
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
A High-throughput Shigella-specific Bactericidal Assay

A High-throughput Shigella-specific Bactericidal Assay

11,132 Views

06:11 min

February 27, 2019

DOI:

06:11 min
February 27, 2019

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Summary

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Here we present a protocol to measure Shigellacidal activity of antibodies in serum. Serum is mixed with bacteria and exogenous complement, incubated, and the reaction mixture is plated on agar plates. Viable bacteria form colonies which are counted, using an automated colony enumerator, and used to determine the bactericidal titer.

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