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Forberedelse og immunfarvning af Myelinating Organotypic cerebellare skive kulturer
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Neuroscienze
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JoVE Journal Neuroscienze
Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures

Forberedelse og immunfarvning af Myelinating Organotypic cerebellare skive kulturer

DOI:
10.3791/59163-v

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09:41 min

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March 20, 2019

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, , , ,
1Sorbonne Université, Inserm, CNRS, Institut du Cerveau et de la Moelle épinière, ICM-GH Pitié-Salpétrière

Capitoli

  • 00:04Titolo
  • 00:36Cerebellum Dissection and Sections Preparation
  • 03:58Slices Culture and Demyelination
  • 04:55Immunohistochemistry
  • 08:15Results: Myelin Immunostaining in Organotypic Cerebellar Slices
  • 09:03Conclusion

Summary

Traduzione automatica

Traduzione automatica

Her vil vi præsentere en metode til at forberede organotypic skive kulturer fra mus lillehjernen og myelinskeden farvning af Immunhistokemi egnet til at undersøge mekanismerne i myelination og remyelination i det centrale nervesystem.

Tags

Myelinating Organotypic Cerebellar Slice CulturesMyelinationRemyelinationCerebellumDissectionImmunostaining

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